Coding
Part:BBa_K5378006:Design
Designed by: pan zhao Group: iGEM24_SMU-GDMU-CHINA (2024-09-25)
tynA-G494S
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 267
Illegal AgeI site found at 522
Illegal AgeI site found at 2074 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
TynA is a periplasmic amine oxidase containing copper and topaquinone cofactors, which converts amines to aldehydes.TynA-G494S discovered by Rottinghaus, A. G. et al converts phenethylamine (PEA) to phenylacetaldehyde (PAG) specifically.
Source
Escherichia coli
References
Reference:Rottinghaus, A. G., Xi, C., Amrofell, M. B., Yi, H., & Moon, T. S. (2022). Engineering ligand-specific biosensors for aromatic amino acids and neurochemicals. Cell systems, 13(3), 204-214.